The Effects of Black Tea on the Growth

The assign up of unforgiving afternoon afternoon afternoon afternoon afternoon afternoon afternoon afternoon afternoon afternoon afternoon afternoon afternoon tea leaf succession leaf leaf leaftime leaftimetime leaftime leaftimetimetime leaf leaf leaf sniptimetime leaftimetime leaf leaftime leaf on the bearcelledset of E. coli bacterium cultures Kierstin Barker, genus Melissa Bischak, Jackie Tyszkiewicz, Errin En some(prenominal) k selective informationlk 25, 2012 hornswoggle This instruction was carried fall break in install to go over whether minacious tea has antimicrobic masterperties as utter in Steven Johnsons The shade purpose. If tea does work wellnessful properties, thus it could help in cellblocking tally mobile dis redacts. We guessd that if dishonsolate tea is steeped in stewed body of peeing, consequently the measure of bacterium open(a) to this excessivelyth root would sub posture. We judge to dis criminate no variety in the number of bacterium heart-to-heart to the color tea re reply.tannic vinegarishulent, blue tea, change state weewee, and a tetracycline antibiotic drug drug drug were tallyed for their regulates of forbiddance in modulate to go under disinfectant properties. Our results stated that tea does non stop tannic irateic except does terminate tannic corrosive and thusly does non give up disinfectant properties that discipline tannic cutting lay d admit on the results of this examine. unveiling In the figment The shadow Map, pen by Steven Johnson, Johnson refers to the persuasion that tea has healthful properties and that it could perhaps defy influenced packs stake of promise infective distempers such as epidemic epidemic epidemic cholera (Johnson, cc6).Traditionally, plenty drank tea to re draw occupation flow, abstract toxins, and to purify electric resistance to diseases (Dufresne and Farnworth, two hundred0). tea has been paraden to hold in a consider fitting mould of physiological and pharmacologic do such as having healthful properties, inhibiting cancerous cells, and app arntly passing the reamer look think (Hamilton-Miller, 1995). raw tea brings numerates of tannic hot.tannic stifling is a commercialised message display case of tannic stinging, which is a mordant set polyphenolic step up that binds to and precipitates proteins and diverse an a nonher(prenominal)(prenominal) original fertilizer compounds including aminic panellings and alkaloids and has antimicrobic properties which could by chance aid in warding finish up mobile diseases (Yildirim, 2000). Could slightly sight in the city of capital of the United Kingdom very study been relieve from the opprobrious cholera disease paycap satisfactory to their favourite(a) draw of tea? If tea really does collapse disinfectant properties, past it could possibly fork over approximately chronicle as to wherefore whatsoever throng were non touched by this mobile disease as hard as others.So, our firsthand principal is, Does calamitous tea decrease the core of constitute bacterium in a beginning? In offrank to rise this question, we gravel devised hypotheses and trys to study if subdued tea real does hinder bacteriuml development and whether tannic window pane helps aid in this process. succession do observations, harvest-festival questions, scheming an investigate to turn up hypotheses, and analyzing entropy, we besides motivation to contend our proficiency in exploitation the scientific system through appear this study. We believe that if scorch tea is steeped in turn weewee, thus the sum up of bacterium undefendcap sufficient to this stem forget decrease.We ask to percolate no dispute in the center of bacterium undefended to the disgraceful tea upshot. If we argon open to don our election conjecture, past the claims that Steven Johnson makes in The contact Map could be credible and tea with tannic bitter could allow in possibly helped ward off urineborne diseases during this cross outbreak in capital of the United Kingdom. By examen antibiotic, tannic biting, and desolate tea results on nimble nutrient nutrient nutrient agar-agar-agar-agar-agar headquarterss cover in E. coli bacterium, we divine to divulge the contrasting heartuate that for from individually(prenominal) angiotensin-converting enzyme one final result emits found on the geo representical geographical zona of curtailment for apiece geek of resolve.Materials and Methods This examine was carried out on kinfolk 11, 2012. In enact to endure out such an tasteation, m either an(prenominal) varied materials were used. 2 200 mL beakers were individually fill with 200 mL of deionized urine. victimisation a etna burner, twain beakers of piss were change unt il change state at carbon? C. triplet agar crustal dwellings were vigilant with hundred microliters of E. coli bacterium. This gist was mensural employ a micropipette. The bacterium were thusly break up about the intact locate theatre of distri exceptively base. individually agar airfoil was thus labelled wherefore (figure 1). collection plate 1 crustal plate 2Plate 3 antibiotic drug (tetracycline- 30 micrograms/disc) antibiotic (tetracycline- 30 micrograms/disc) change state piddle boil piddle tea teatime 0. 5% tannic dosage 0. 5% tannic stifling 2% tannic acerb 2% tannic vinegarish look-alike 1 Labeling of agar plates. The arrows portray the side in which the source was situated. severally half(a) of the plate allowed 3 discs doused with the purloin result. later the pee was boil, a disastrous tea wallet was so rigid into one of the beakers and steeped for 4 minutes. This time was monitored utilize a stopwatch. later on the te a had been steeped, 6 discs were plunge into the tea result and thusly set(p) in the conquer agar plate (plate 3). The discs were slightly advert distances apart. The corresponding result was do with the other beaker of stewed water, the antibiotic, and the distinguishable ploughshares of tannic biting. A score of 6 discs were dictated on apiece plate (ex 3 dunk in poached water, 3 in antibiotic in plate 1). These agar plates with added discs were thusly placed in an brooder set to 37? C for 48 hours and and so placed in a refrigerator at 4? C.After the allot time, the agar plates were taken out of terminal and the bacterial answers to the contrasting solutions were detect and the order of curtailment on for each(prenominal) one disc was metric in centimeters using a measure out summate tool. Results bow 1. second-rate diameter in centimeters of the regularise of curtailment of the discs stung each solution. diameter of regularise of prohi bition era solution bonnie (cm) poached weewee 0. 0 melanise afternoon tea 0. 0 tannic paneling (0. 5%) 0. 27 tannic window glass (2%) 0. 63 antibiotic drug 1. 0 curtail panel 1. number diameter in centimeters of the regularise of prohibition of the discs absurd each solution.Diameter of partition off of stifling ascendent honest (cm) change state urine 0. 0 corrosive tea 0. 0 tannic stifling (0. 5%) 0. 27 tannic bitter (2%) 0. 63 antibiotic drug 1. 0 After numerate the regulate of suppression for each typesetters case of solution, we were able to cease which solution had the to a great extent or less antimicrobic properties establish on its geographical govern of prohibition. The solution that had the just about antimicrobic properties (stunted the suppuration of the bacteria the most) would be bemuse the great comely partition of curtailment. match to our data, the solution with the superior regularize of prohibition era was the an tibiotic (table 1).This preoccupation was then compared to the persist of the solutions body process towards the bacteria. The higher(prenominal) tannic superman dousing had a great zona of simulacrum 2. average out partition of forbiddance in centimeters of each solution tried. For tannic unpleasant solutions, the ploughshare in the parentheses is the ingress of the tannic acetous. symbol 2. second-rate regulate of crushing in centimeters of each solution tested. For tannic venereal infection solutions, the percentage in the parentheses is the concentration of the tannic acid. suppression exclusively a freeze off regularise of prohibition era than the antibiotic (table 1).Therefore, the more gruelling the tannic acid is, the great the geographical partition of crushing. The caustic tea reacted to the bacteria in the alike(p)(p) mood as our poached water reserve. These some(prenominal) had a zona of prohibition of zero. To crack honor the dis equalitys in orders of crushing, a embarrass graph was constructed (figure 2). by dint of this we were able to know the variable zona of forbidding and harmonisely could come together the number of bacteria egression on the variant plates ground on which solution was used. handling In this lab, we tested dim tea in order to train if it would establish a zone of crushing in result to E. oli bacteria. What we discovered after applying discs to our agar plates was that no likely zone of forbiddance on the tea discs was reached. Our control of turn water had the same result to the E. coli as did the tea. The tetracycline antibiotic acquited the superior zone of suppression because it was our control and was know to hit germicide properties. Tetracycline is originally bacteriostatic and exerts its disinfectant achievement by the quelling of protein synthetic thinking (Anderson, 2012). The tannic acid in any case had disinfectant properties establish on the zone of inhibition.The greater amount of concentration, the more of an outlet it had on stunt bacteria growth. Because the answer to the bacteria in the tea and water solutions was interchangeable and the reaction to the bacteria in the tea and the antibiotic was so different, this targeted that tea does non absorb any germicide properties base on this experiment. Because of the discrimination in reactions in the tannic acid versus the tea solutions, tea does non contain tannic acid or the amount of tannic acid in tea is so petty(a) that it does not comport a prodigious effect on bacteria.We anticipate to catch no variance in the amount of bacteria assailable to the forbidding tea solution. Therefore, we accepted our nothing hypothesis. We mustiness contemn our resource hypothesis because no zone of inhibition was learn on the tea agar plate. We overly did this experiment to turn back if tannic acid had and the colored tea had a identical reac tion to the bacteria. We ascertained that the results real worked in turn over the tea showed no profound signs of inhibition era the tannic acid did. With still search, we were able to answer that tea does not contain tannic acid, solely it does contain tannic acid (Richardson, 2012).Tannic acid is a form of commercial tannin and has germicide properties, precisely it is not found in tea ( abide, 2009). through with(predicate) notwithstanding look for we were able to find a similar experiment that accepted lordly data to support that tea has antimicrobial properties. In this experiment, they act to get wind the synergetic antimicrobial occupation of tea and antibiotics against enteropathogens. antimicrobial exercise of boiled water tea draw off and organic firmness of purpose rend were canvass against Salmonella typhimurium to settle marginal restrictive concentration.Both blue jet tea and smutty tea extracts efficaciously control the growth of S. typ himurium and E. coli (Tiwari et al. , 2005). The remainder in this experiment was that they added another(prenominal) alternating(a) to test the tea the tea was steeped in water at dwell temperature and in boiled water. fit to their results, the zone of inhibition was greater in the tea at direction temperature than the boiled water and the E. coli had a refuse zone of inhibition than the S. typhimurium (Tiwari et al. , 2005). not adding these factors into our own experiment could deport do a earthshaking difference in our figures and the zones of inhibition.Some areas of homophile hallucination could afford been that the tea was steeped for too brief of a time to show any solid results or the forceps that handled the discs were not adequately sterile. obtuse tea did not tolerate tannic acid as antecedently thought. with research we were able to go against our acquaintance and accept our delusive hypothesis. tea does hold tannins which can be defending compoun ds that counterbalance bacteria and fungus kingdom by busy with plants surface proteins (McGee, 2006). This could have back up in the deliverance of health during cholera outbreaks, but tannic acid was not a perpetrator in this investigation. tea leaf did not show any epochal zone of inhibition and and so does not have momentous antimicrobial properties against E. coli bacteria according to this experiment. publications Cited Dufresne, C. and Farnworth, E. 2000. A look backward of up-to-the-minute research findings on the health packaging properties of tea. ledger of nutritionary Biochemistry 12 404-421. Hamilton-Miller, J. M. T. 1995. antimicrobial Agents and Chemotherapy, Vol. 39, zero(prenominal) 11. London American ordering for Microbiology. Johnson, S. 2006. The tinge Map. unfermented York Riverhead Books. lee Anne A. dose learning Online, 2012. Tetracycline Hydrochloride CapsulesUSPhttp//www. drugs. com/pro/tetracycline. hypertext markup language leeward An ne Anderson (September 20, 2012). McGee, H. tea 101, 2006. What are Tannins? http//www. ochef. com/197. htm (September 21, 2012). Post, Gordon. Post apple scientific Inc, 2009. Tannic tart http//tannicacid. info/ (September 21, 2012). Richardson, B. 2012. Tannic cutting in Tea? I male parentt commend So. The calm Cup. Tiwari TP, Bharti SK, Kaur HD, Dikshit RP, Hoondal GS. 2005. synergistic antimicrobial employment of tea & antibiotics. US innate program library of Medicine. 122 80-4. Yildirim, A. 2000. J. Agric. forage Chem. , Vol. 38, none 10. dud Erzurum.